| Peer-Reviewed

Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis

Received: 20 February 2018     Accepted: 6 March 2018     Published: 23 March 2018
Views:       Downloads:
Abstract

The morphology transformation of giant vesicles consisting of amphiphilic poly(methacrylic acid)- block-poly(methyl methacrylate-random-methacrylic acid-random-3-sulfopropyl methacrylate potassium salt), PMAA-b-P(MMA-r-MAA-r-SpMA), was investigated using poly(allylamine hydrochloride) (PAH) as an artificial model of a membrane protein for endocytosis. The polymerization-induced self-assembly of the PMAA-b-P(MMA-r-MAA-r-SpMA) using the nitroxide-mediated photo-controlled/living radical polymerization technique produced spherical vesicles in the absence of PAH, while it provided a fused sheet in its presence at a 1.0 molar ratio of the allylamine hydrochloride unit (AH) to the SpMA unit. It was suggested that the PAH connected the SpMA units by an electrostatic interaction. The fused sheet changed into combined vesicles as the AH/SpMA ratio increased, and at AH/SpMA = 10.0, the morphology was transformed into spherical vesicles much smaller than the original vesicles. The morphology transformation by soaking the original spherical vesicles in a PAH solution demonstrated that the PAH caused the division of the vesicles into much smaller spherical vesicles.

Published in Colloid and Surface Science (Volume 3, Issue 1)
DOI 10.11648/j.css.20180301.12
Page(s) 6-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Giant Vesicles, Amphiphilic Diblock Copolymer, Photo NMP-Induced Self-assembly, Morphology Transformation, Vesicle Division, Polyelectrolyte, Electrostatic Interaction

References
[1] H. Sprong, P. Sluijs, and G. Meer, Nature Rev. Mol. Cell Biol. 2001, 2, 504-513.
[2] B. L. Deatherage and B. T. Cookson, Infection and Immunity, 2012, 80, 1948-1957.
[3] E. J. Blott and G. M. Griffiths, Nature Rev. Mol. Cell Biol. 2002, 3, 122-131.
[4] A. Sorkin and M. Zastrow, Nature Rev. Mol. Cell Biol. 2002, 3, 600-614.
[5] S. D. Conner and S. L. Schmid, Nature, 2003, 422, 37-44.
[6] J. S. Bonifacino and B. S. Glick, Cell, 2004, 116, 153-166.
[7] H. Ewers and A. Helenius, Cold Spring Harbor Perspectives in Biology, 2011, 3, a004721, 1-14.
[8] S. Mukherjee, R. N. Ghosh, and F. R. Maxfield, Phycolog. Rev. 1997, 77, 759-803.
[9] R. G. W. Anderson, B. A. Kamen, K. G. Rothberg, and S. W. Lacey, Science, 1992, 255, 410-411
[10] C. Mineo and R. G. W. Anderson, Histochem. Cell Biol. 2001, 116, 109-118.
[11] N. J. Severs, J. Cell Sci. 1988, 90, 341-348.
[12] K. G. Rothberg, J. E. Heuser, W. C. Donzell, Y-S. Ying, J. R. Glenney, and R. G. W. Anderson, Cell, 1992, 68, 673-682.
[13] T. V. Kurzchalia, P. Dupree, R. G. Parton, R. Kellner, H. Virta, M. Lehnert, and K. Simons, J. Cell Biol. 1992, 118, 1003-1014.
[14] T. M. Williams and M. P. Lisanti, Genome Biol. 2004, 5, 214.1-214.8.
[15] E. Yoshida, Colloid Polym. Sci. 2013, 291, 2733-2739.
[16] E. Yoshida, Colloid Polym. Sci. 2015, 293, 649-653.
[17] E. Yoshida, Chem Xpress, 2017, 10, 118, 1-11.
[18] E. Yoshida, Colloid Polym. Sci. 2015, 293, 2437-2443.
[19] E. Yoshida, Colloid Polym. Sci. 2015, 293, 249-256.
[20] E. Yoshida, Colloid Polym. Sci. 2015, 293, 3641-3648.
[21] E. Yoshida, Colloid Polym. Sci. 2014, 292, 2555-2561.
[22] E. Yoshida, Colloid Polym. Sci. 2014, 292, 763-769.
[23] E. Yoshida, Cogent Chemistry, 2016, 2, 1212319, 1-16.
[24] E. Yoshida, Colloid Polym. Sci. 2015, 293, 1835-1840.
[25] E. Yoshida, Supramol. Chem. 2015, 27, 274-280.
[26] E. Yoshida, Open J. Polym. Chem. 2013, 3, 16-22.
[27] E. Yoshida, Colloid Polym. Sci. 2016, 294, 1857-1863.
[28] E. Yoshida, Colloid Polym. Sci. 2015, 293, 1841-1845.
[29] J. D. Mendelsohn, S. Y. Yang, J. A. Hiller, A. I. Hochbaum, M. F. Rubner, Biomacromolecules, 2003, 4, 96-106.
[30] E. Yoshida, Colloid Polym. Sci. 2010, 288, 1321-1325.
[31] E. Yoshida, Colloid Polym. Sci. 2013, 291, 993-1000.
[32] T. Miyazawa, T. Endo, S. Shiihashi, M. Ogawara, J. Org. Chem. 1985, 50, 1332-1334.
[33] E. Yoshida ISRN Polym. Sci., 2012, 102186, 1-6.
[34] S. Kobayashi, H. Uyama, I. Yamamoto, Y. Matsumoto. Polym. J. 22 (1990), 759-761.
[35] E. Yoshida, Colloid Polym. Sci. 2015, 293, 1275-1280.
[36] E. Yoshida, Colloid Polym. Sci. 2014, 292, 1463-1468.
Cite This Article
  • APA Style

    Eri Yoshida. (2018). Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis. Colloid and Surface Science, 3(1), 6-11. https://doi.org/10.11648/j.css.20180301.12

    Copy | Download

    ACS Style

    Eri Yoshida. Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis. Colloid Surf. Sci. 2018, 3(1), 6-11. doi: 10.11648/j.css.20180301.12

    Copy | Download

    AMA Style

    Eri Yoshida. Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis. Colloid Surf Sci. 2018;3(1):6-11. doi: 10.11648/j.css.20180301.12

    Copy | Download

  • @article{10.11648/j.css.20180301.12,
      author = {Eri Yoshida},
      title = {Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis},
      journal = {Colloid and Surface Science},
      volume = {3},
      number = {1},
      pages = {6-11},
      doi = {10.11648/j.css.20180301.12},
      url = {https://doi.org/10.11648/j.css.20180301.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.css.20180301.12},
      abstract = {The morphology transformation of giant vesicles consisting of amphiphilic poly(methacrylic acid)- block-poly(methyl methacrylate-random-methacrylic acid-random-3-sulfopropyl methacrylate potassium salt), PMAA-b-P(MMA-r-MAA-r-SpMA), was investigated using poly(allylamine hydrochloride) (PAH) as an artificial model of a membrane protein for endocytosis. The polymerization-induced self-assembly of the PMAA-b-P(MMA-r-MAA-r-SpMA) using the nitroxide-mediated photo-controlled/living radical polymerization technique produced spherical vesicles in the absence of PAH, while it provided a fused sheet in its presence at a 1.0 molar ratio of the allylamine hydrochloride unit (AH) to the SpMA unit. It was suggested that the PAH connected the SpMA units by an electrostatic interaction. The fused sheet changed into combined vesicles as the AH/SpMA ratio increased, and at AH/SpMA = 10.0, the morphology was transformed into spherical vesicles much smaller than the original vesicles. The morphology transformation by soaking the original spherical vesicles in a PAH solution demonstrated that the PAH caused the division of the vesicles into much smaller spherical vesicles.},
     year = {2018}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Morphology Transformation of Giant Vesicles by a Polyelectrolyte for an Artificial Model of a Membrane Protein for Endocytosis
    AU  - Eri Yoshida
    Y1  - 2018/03/23
    PY  - 2018
    N1  - https://doi.org/10.11648/j.css.20180301.12
    DO  - 10.11648/j.css.20180301.12
    T2  - Colloid and Surface Science
    JF  - Colloid and Surface Science
    JO  - Colloid and Surface Science
    SP  - 6
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2578-9236
    UR  - https://doi.org/10.11648/j.css.20180301.12
    AB  - The morphology transformation of giant vesicles consisting of amphiphilic poly(methacrylic acid)- block-poly(methyl methacrylate-random-methacrylic acid-random-3-sulfopropyl methacrylate potassium salt), PMAA-b-P(MMA-r-MAA-r-SpMA), was investigated using poly(allylamine hydrochloride) (PAH) as an artificial model of a membrane protein for endocytosis. The polymerization-induced self-assembly of the PMAA-b-P(MMA-r-MAA-r-SpMA) using the nitroxide-mediated photo-controlled/living radical polymerization technique produced spherical vesicles in the absence of PAH, while it provided a fused sheet in its presence at a 1.0 molar ratio of the allylamine hydrochloride unit (AH) to the SpMA unit. It was suggested that the PAH connected the SpMA units by an electrostatic interaction. The fused sheet changed into combined vesicles as the AH/SpMA ratio increased, and at AH/SpMA = 10.0, the morphology was transformed into spherical vesicles much smaller than the original vesicles. The morphology transformation by soaking the original spherical vesicles in a PAH solution demonstrated that the PAH caused the division of the vesicles into much smaller spherical vesicles.
    VL  - 3
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi, Japan

  • Sections